IMG Electron Microscopy Core Facility

0555851 - ÚMG 2022 RIV CZ eng O - Others
Filimonenko, Vlada - Pinkas, Dominik - Vlčák, Erik - Raabová, Helena - Hozák, Pavel
IMG Electron Microscopy Core Facility.
2021
R&D Projects: GA MŠMT(CZ) LM2018129; GA MŠMT(CZ) EF16_013/0001775; GA MŠMT(CZ) EF18_046/0016045; GA TA ČR(CZ) TE01020118
Research Infrastructure: Czech-BioImaging II - 90129
Institutional support: RVO:68378050
Keywords : Transmission lectron microscopy * sample preparation * Czech-BioImaging * Euro-BioImgaging
OECD category: Cell biology

The Electron Microscopy Core Facility provides expertise and cutting-edge equipment for a broad range of biological sample preparation and ultrastructural imaging techniques. The core facility deals with various biological samples: human and animal cell cultures, plant and animal tissues, worms, microorganisms, lipid micelles, isolated DNA. The sample preparation techniques include routine chemical fixation and resin embedding, negative staining, cryofixation using plunge-freezing or high-pressure freezing, freeze-substitution, cryosectioning, freeze fracture replica labeling, pre- and post-embedding immunolabeling, the list being lately extended by cryoCLEM using specialized microscope.
High-pressure freezer, two automatic freeze-substitution machines, cryo-ultramicrotomes, automated plunge-freezer, freeze-fracture replica making device, cryoCLEM microscope, as well as additional wet lab equipment are available. Transmission electron microscopes (TEM) installed in the end of 2019 include a standard 120 kV instrument for routine observation and an advanced 200 kV S/TEM providing the possibility of high-resolution TEM, 3D electron tomography, cryoelectron microscopy and EDS elemental analysis.
The team has a long expertise in the development and optimization of sample preparation techniques, including fruitful collaborations with companies providing equipment for electron microscopy.
The Electron Microscopy Core Facility is part of the IMG Czech-BioImaging node and Prague Euro-BioImaging node. We provide open access to our technologies and expertise and are ready to welcome users from all fields.
Permanent Link: http://hdl.handle.net/11104/0330308