Exosomes produced by melanoma cells significantly influence the biological properties of normal and cancer-associated fibroblasts

0555562 - ÚMG 2023 RIV DE eng J - Journal Article
Strnadová, K. - Pfeiferová, Lucie - Přikryl, P. - Dvořánková, B. - Vlčák, Erik - Frýdlová, J. - Vokurka, M. - Novotný, Jiří - Šáchová, Jana - Hradilová, Miluše - Brábek, J. - Šmigová, J. - Rösel, D. - Smetana, K. Jr. - Kolář, Michal - Lacina, L.
Exosomes produced by melanoma cells significantly influence the biological properties of normal and cancer-associated fibroblasts.
Histochemistry and Cell Biology. Roč. 157, č. 2 (2022), s. 153-172. ISSN 0948-6143. E-ISSN 1432-119X
R&D Projects: GA MŠMT(CZ) EF16_019/0000785; GA MŠMT(CZ) LM2018129; GA MŠMT(CZ) EF18_046/0016045; GA MŠMT(CZ) EF16_013/0001775; GA ČR(CZ) GA19-05048S
Institutional support: RVO:68378050
Keywords : Melanoma * Cancer-associated fibroblasts * Exosomes * il-6 * il-8 * Proinflammatory cytokine
OECD category: Cell biology
Impact factor: 2.3, year: 2022
Method of publishing: Open access
https://link.springer.com/article/10.1007/s00418-021-02052-2

The incidence of cutaneous malignant melanoma is increasing worldwide. While the treatment of initial stages of the disease is simple, the advanced disease frequently remains fatal despite novel therapeutic options . This requires identification of novel therapeutic targets in melanoma. Similarly to other types of tumours, the cancer microenvironment plays a prominent role and determines the biological properties of melanoma. Importantly, melanoma cell-produced exosomes represent an important tool of intercellular communication within this cancer ecosystem. We have focused on potential differences in the activity of exosomes produced by melanoma cells towards melanoma-associated fibroblasts and normal dermal fibroblasts. Cancer-associated fibroblasts were activated by the melanoma cell-produced exosomes significantly more than their normal counterparts, as assessed by increased transcription of genes for inflammation-supporting cytokines and chemokines, namely IL-6 or IL-8. We have observed that the response is dependent on the duration of the stimulus via exosomes and also on the quantity of exosomes. Our study demonstrates that melanoma-produced exosomes significantly stimulate the tumour-promoting proinflammatory activity of cancer-associated fibroblasts. This may represent a potential new target of oncologic therapy .
Permanent Link: http://hdl.handle.net/11104/0330026