Epigenomic Landscape of Lyme Disease Spirochetes Reveals Novel Motifs

0555231 - BC 2022 RIV US eng J - Journal Article
Wachter, J. - Martens, C. - Barbian, K. - Rego, Ryan O. M. - Rosa, P.
Epigenomic Landscape of Lyme Disease Spirochetes Reveals Novel Motifs.
mBio. Roč. 12, č. 3 (2021), č. článku e01288-21. ISSN 2161-2129. E-ISSN 2150-7511
Institutional support: RVO:60077344
Keywords : Borrelia * Lyme disease * methylation
OECD category: Microbiology
Impact factor: 7.786, year: 2021
Method of publishing: Open access
https://journals.asm.org/doi/10.1128/mBio.01288-21

Borrelia burgdorferi, the etiological agent of Lyme disease, persists in nature through an enzootic cycle consisting of a vertebrate host and an Ixodes tick vector. The sequence motifs modified by two well-characterized restriction/modification (R/M) loci of B. burgdorferi type strain B31 were recently described, but the methylation profiles of other Lyme disease Borrelia bacteria have not been characterized. Here, the methylomes of B. burgdorferi type strain B31 and 7 clonal derivatives, along with B. burgdorferi N40, B. burgdorferi 297, B. burgdorferi CA-11, B. afzelii PKo, B. afzelii BO23, and B. garinii PBr, were defined through PacBio single-molecule realtime (SMRT) sequencing. This analysis revealed 9 novel sequence motifs methylated by the plasmid-encoded restriction/modification enzymes of these Borrelia strains. Furthermore, while a previous analysis of B. burgdorferi B31 revealed an epigenetic impact of methylation on the global transcriptome, the current data contradict those findings, our analyses of wild-type B. burgdorferi B31 revealed no consistent differences in gene expression among isogenic derivatives lacking one or more restriction/ modification enzymes.
Permanent Link: http://hdl.handle.net/11104/0329758