Development of an Albumin-Polymer Bioconjugate via Covalent Conjugation and Supramolecular Interactions

0555120 - ÚOCHB 2023 RIV US eng J - Journal Article
Xu, Y. D. - Tian, L. - Lai, R. Y. - Li, Z. - Procházková, Eliška - Ho, J. - Stenzel, M. H.
Development of an Albumin-Polymer Bioconjugate via Covalent Conjugation and Supramolecular Interactions.
Bioconjugate Chemistry. Roč. 33, č. 2 (2022), s. 321-332. ISSN 1043-1802. E-ISSN 1520-4812
Grant - others:AV ČR(CZ) MSM200551801
Program: Program na podporu mezinárodní spolupráce začínajících výzkumných pracovníků
Institutional support: RVO:61388963
Keywords : human serum albumin * half-life extension * binding interaction
OECD category: Analytical chemistry
Impact factor: 4.7, year: 2022
Method of publishing: Limited access
https://doi.org/10.1021/acs.bioconjchem.1c00536

Preexisting serum albumin-polymer bioconjugates have been formed either through covalent conjugation or supramolecular interactions. However, the viability of producing a bioconjugate where both covalent conjugation and supramolecular interactions have been adopted is yet to be explored. In this work, the noncovalent interaction of two polymers bearing fatty acidbased end-functionalities were compared and the superior binder was carried forward for testing with serum albumin that possessed a polymer conjugated to its Cys34 residue. The studies demonstrated that an albumin-polymer bioconjugate equipped with polymers via both covalent and supramolecular interactions can be successfully achieved.
Permanent Link: http://hdl.handle.net/11104/0331097