NFAT signaling in human mesenchymal stromal cells affects extracellular matrix remodeling and antifungal immune responses

0554898 - BFÚ 2022 RIV US eng J - Journal Article
Tidu, F. - De Zuani, M. - Jose, S. - Bendíčková, K. - Kubala, Lukáš - Caruso, F. - Cavalieri, F. - Forte, G. - Frič, J.
NFAT signaling in human mesenchymal stromal cells affects extracellular matrix remodeling and antifungal immune responses.
iScience. Roč. 24, č. 6 (2021), č. článku 102683. E-ISSN 2589-0042
Institutional support: RVO:68081707
Keywords : toll-like receptors * beta-glucan recognition * c-type lectin * stem-cells * hyaluronan degradation * activation
OECD category: Biophysics
Impact factor: 6.107, year: 2021
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S2589004221006519?via%3Dihub#!

Mesenchymal stromal cells (MSCs) combined with calcineurin-nuclear factor of activated T cell (CN-NFAT) inhibitors are being tested as a treatment for graft-versus-host disease (GvHD). The immunosuppressive properties of MSCs seem beneficial, however, their response during fungal infection, which is an important cause of mortality in patients with GvHD, is unknown. We report that MSCs phagocytose the fungal component zymosan, resulting in phosphorylation of spleen tyrosine kinase (Syk), increase in cytosolic calcium levels, and ultimately, increase in NFAT1 nuclear translocation. RNA sequencing analysis of zymosan-treated MSCs showed that CN-NFAT inhibition affects extracellular matrix (ECM) genes but not cytokine expression that is under the control of the NF-kappa B pathway. When coculturing MSCs or decellularized MSC-ECM with human peripheral blood mononuclear cells (PBMCs), selective NFAT inhibition in MSCs decreased cytokine expression by PBMCs. These findings reveal a dual mechanism underlying the MSC response to zymosan: while NF-kappa B directly controls inflammatory cytokine expression, NFAT impacts immune-cell functions by regulating ECM remodeling.
Permanent Link: http://hdl.handle.net/11104/0329515