Analyses of viral genomes for G-quadruplex forming sequences reveal their correlation with the type of infection

0554894 - BFÚ 2022 RIV FR eng J - Journal Article
Bohalova, Natalia - Cantara, Alessio - Bartas, M. - Kaura, P. - Šťastný, J. - Pečinka, P. - Fojta, Miroslav - Mergny, Jean-Louis - Brázda, Václav
Analyses of viral genomes for G-quadruplex forming sequences reveal their correlation with the type of infection.
Biochimie. Roč. 186, JUL 2021 (2021), s. 13-27. ISSN 0300-9084. E-ISSN 1638-6183
R&D Projects: GA ČR(CZ) GA18-15548S; GA MŠMT EF15_003/0000477
Institutional support: RVO:68081707
Keywords : rna * replication * promoter * viruses * binding * zika * G-quadruplex
OECD category: Biochemistry and molecular biology
Impact factor: 4.372, year: 2021
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S0300908421000961?via%3Dihub

G-quadruplexes contribute to the regulation of key molecular processes. Their utilization for antiviral therapy is an emerging field of contemporary research. Here we present comprehensive analyses of the presence and localization of putative G-quadruplex forming sequences (PQS) in all viral genomes currently available in the NCBI database (including subviral agents). The G4Hunter algorithm was applied to a pool of 11,000 accessible viral genomes representing 350 Mbp in total. PQS frequencies differ across evolutionary groups of viruses, and are enriched in repeats, replication origins, 50UTRs and 30UTRs. Importantly, PQS presence and localization is connected to viral lifecycles and corresponds to the type of viral infection rather than to nucleic acid type, while viruses routinely causing persistent infections in Metazoa hosts are enriched for PQS, viruses causing acute infections are significantly depleted for PQS. The unique localization of PQS identifies the importance of G-quadruplex-based regulation of viral replication and life cycle, providing a tool for potential therapeutic targeting. (C) 2021 The Authors. Published by Elsevier B.V.
Permanent Link: http://hdl.handle.net/11104/0329513