Dual-color dSTORM imaging and ThunderSTORM image reconstruction and analysis to study the spatial organization of the nuclear phosphatidylinositol phosphates

0554635 - ÚMG 2022 RIV NL eng J - Journal Article
Hoboth, Peter - Šebesta, O. - Sztacho, Martin - Castano, Enrique - Hozák, Pavel
Dual-color dSTORM imaging and ThunderSTORM image reconstruction and analysis to study the spatial organization of the nuclear phosphatidylinositol phosphates.
MethodsX. Roč. 8, May (2021), č. článku 101372. E-ISSN 2215-0161
R&D Projects: GA MŠMT(CZ) LM2018129; GA MŠMT(CZ) EF16_013/0001775; GA ČR GA19-05608S; GA ČR(CZ) GA18-19714S; GA MŠMT LTC19048; GA MŠMT LTC20024; GA MŠMT(CZ) ED1.1.00/02.0109
Research Infrastructure: Czech-BioImaging II - 90129
Institutional support: RVO:68378050
Keywords : Immunofluorescence * Wide-field microscopy * Super-resolution microscopy * ImageJ * Nearest neighbor distance * Cell nucleus * Nuclear architecture * Nuclear speckles * son * RNA polymerase II * Fibrillarin
OECD category: Cell biology
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S2215016121001655?via%3Dihub

Single molecule localization microscopy (SMLM) provided an unprecedented insight into the sub-nuclear organization of proteins and nucleic acids but apart from the nuclear envelope the role of the nuclear lipids in the functional organization of the cell nucleus was less studied. Nevertheless, nuclear lipids and specifically phosphatidylinositol phosphates (PIPs) play increasingly evident roles in gene expression. Therefore, here we provide the SMLM-based approach for the quantitative evaluation of the nuclear PIPs distribution while preserving the context of nuclear architecture. Specifically, on the example of phosphatidylinositol 4,5-bisphosphate (PIP2) we have:
Permanent Link: http://hdl.handle.net/11104/0329333