Pseurotin D Induces Apoptosis through Targeting Redox Sensitive Pathways in Human Lymphoid Leukemia Cells

0554463 - BFÚ 2022 RIV CH eng J - Journal Article
Mosejová, Eva - Bosnjakovic, Rebeka - Kubala, Lukáš - Vašíček, Ondřej
Pseurotin D Induces Apoptosis through Targeting Redox Sensitive Pathways in Human Lymphoid Leukemia Cells.
Antioxidants. Roč. 10, č. 10 (2021), č. článku 1576. E-ISSN 2076-3921
R&D Projects: GA ČR(CZ) GA17-18858S
Institutional support: RVO:68081707
Keywords : angiogenesis * mitochondria * azaspirene * activation * synerazol
OECD category: Biochemistry and molecular biology
Impact factor: 7.675, year: 2021
Method of publishing: Open access
https://www.mdpi.com/2076-3921/10/10/1576

Chronic lymphocytic leukemia (CLL) is the most prevalent lymphoid malignancy in many geographical regions of the world. Pseurotin D, a secondary metabolite of fungi, represents a group of bioactive natural products with a newly ascribed range of interesting biological activities. The purpose of this study was to bring new insights into the mechanism behind the effects of pseurotin D on MEC-1 cells as a representative CLL cell line, with a particular focus on selected signaling pathways important in the proliferation of cells and targeting mitochondrial metabolism. Our results showed that pseurotin D was able to significantly inhibit the proliferation of MEC-1 cells and arrested them in the G2/M cell cycle phase. In addition, pseurotin D was able to induce apoptosis. We found that all of these effects were associated with a change in mitochondrial membrane potential and the production of mitochondrial reactive oxygen species (ROS). We showed for the first time that pseurotin D suppresses MEC-1 cell proliferation and induces apoptotic cell death via induction of the collapse of the mitochondria respiratory chain and the ROS-related caspase pathway. Our results show the pseurotins family as promising compounds which could serve as a basis for the development of new compounds in the treatment of lymphoma./p
Permanent Link: http://hdl.handle.net/11104/0329175