Enigmatic Evolutionary History of Porphobilinogen Deaminase in Eukaryotic Phototrophs

0554417 - BC 2022 RIV CH eng J - Journal Article
Oborník, Miroslav
Enigmatic Evolutionary History of Porphobilinogen Deaminase in Eukaryotic Phototrophs.
Biology. Roč. 10, č. 5 (2021), č. článku 386. E-ISSN 2079-7737
R&D Projects: GA ČR(CZ) GA21-03224S; GA MŠMT(CZ) EF16_019/0000759
Institutional support: RVO:60077344
Keywords : sequence alignment * origin * mitochondria * pathway * ferrochelatase * phylogeny * heme * tree * porphobilinogen deaminase * hydroxymethylbilane synthase * heme biosynthesis * evolution * mitochondrion * gene replacement * horizontal gene transfer
OECD category: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Impact factor: 5.168, year: 2021
Method of publishing: Open access
https://www.mdpi.com/2079-7737/10/5/386

In most eukaryotic phototrophs, the entire heme synthesis is localized to the plastid, and enzymes of cyanobacterial origin dominate the pathway. Despite that, porphobilinogen deaminase (PBGD), the enzyme responsible for the synthesis of hydroxymethybilane in the plastid, shows phylogenetic affiliation to α-proteobacteria, the supposed ancestor of mitochondria. Surprisingly, no PBGD of such origin is found in the heme pathway of the supposed partners of the primary plastid endosymbiosis, a primarily heterotrophic eukaryote, and a cyanobacterium. It appears that α-proteobacterial PBGD is absent from glaucophytes but is present in rhodophytes, chlorophytes, plants, and most algae with complex plastids. This may suggest that in eukaryotic phototrophs, except for glaucophytes, either the gene from the mitochondrial ancestor was retained while the cyanobacterial and eukaryotic pseudoparalogs were lost in evolution, or the gene was acquired by non-endosymbiotic gene transfer from an unspecified α-proteobacterium and functionally replaced its cyanobacterial and eukaryotic counterparts
Permanent Link: http://hdl.handle.net/11104/0329126