Topological Analysis of gamma H2AX and MRE11 Clusters Detected by Localization Microscopy during X-ray-Induced DNA Double-Strand Break Repair

0554412 - BFÚ 2022 RIV CH eng J - Journal Article
Hahn, H. - Neitzel, Ch. - Kopečná, Olga - Heermann, D.W. - Falk, Martin - Hausmann, M.
Topological Analysis of gamma H2AX and MRE11 Clusters Detected by Localization Microscopy during X-ray-Induced DNA Double-Strand Break Repair.
Cancers (Basel). Roč. 13, č. 21 (2021), č. článku 5561. E-ISSN 2072-6694
R&D Projects: GA ČR GC20-04109J
Institutional support: RVO:68081707
Keywords : histone h2ax phosphorylation * complex cell responses * homologous recombination * nanoscopy techniques * pathway choice
OECD category: Oncology
Impact factor: 6.575, year: 2021
Method of publishing: Open access
https://www.mdpi.com/2072-6694/13/21/5561

Simple Summary: Super-resolution single-molecule localization microscopy (SMLM) allows the detection of cluster formation of phosphorylation sites and recruited repair proteins (e.g., MRE11) around DNA double-strand breaks induced by ionizing radiation. The spatial arrangement of these targets can be described by distance frequency distributions, which can be used as a measure for cluster size and the ratio of cluster numbers per specimen analyzed. For the analysis of the shape of clusters and their topology and similarity, persistent homology was applied and the features were compared along the DNA repair process for two different cell lines (MCF-7 breast cancer cells and CCD-1059SK human skin fibroblasts). The similarity values for components and holes were averaged for each repair time point post-irradiation so that it was possible to compare not only clusters with each other but also cell specimens of different repair periods with each other. It was shown that after X-ray irradiation, these similarity values were high for gamma H2AX clusters in the early repair phase, while MRE11 clusters revealed an increased similarity at later time points.
Permanent Link: http://hdl.handle.net/11104/0329109