Community Parameters and Genome-Wide RAD-Seq Loci of Ceratothoa oestroides Imply Its Transfer between Farmed European Sea Bass and Wild Farm-Aggregating Fish

0554312 - BC 2022 RIV CH eng J - Journal Article
Mladineo, Ivona - Hrabar, J. - Trumbic, Z. - Manousaki, T. - Tsakogiannis, A. - Taggart, J. - Tsigenopoulos, C. S.
Community Parameters and Genome-Wide RAD-Seq Loci of Ceratothoa oestroides Imply Its Transfer between Farmed European Sea Bass and Wild Farm-Aggregating Fish.
Pathogens. Roč. 10, č. 2 (2021), č. článku 100. E-ISSN 2076-0817
EU Projects: European Commission(XE) 634429 - ParaFishControl
Institutional support: RVO:60077344
Keywords : piscirickettsia-salmonis * dicentrarchus-labrax * population genomics * isopod parasites * atlantic * aquaculture * resistance * bream * association * cymothoidae * Ceratothoa oestroides * Dicentrarchus labrax * mean abundance * mean intensity * parasite transfer * prevalence * RAD-Seq
OECD category: Microbiology
Impact factor: 4.531, year: 2021
Method of publishing: Open access
https://www.mdpi.com/2076-0817/10/2/100

Wild fish assemblages that aggregate within commercial marine aquaculture sites for feeding and shelter have been considered as a primary source of pathogenic parasites vectored to farmed fish maintained in net pens at an elevated density. In order to evaluate whether Ceratothoa oestroides (Isopoda, Cymothoidae), a generalist and pestilent isopod that is frequently found in Adriatic and Greek stocks of farmed European sea bass (Dicentrarchus labrax), transfers between wild and farmed fish, a RAD-Seq (restriction-site-associated DNA sequencing)-mediated genetic screening approach was employed. The double-digest RAD-Seq of 310 C. oestroides specimens collected from farmed European sea bass (138) and different wild farm-aggregating fish (172) identified 313 robust SNPs that evidenced a close genetic relatedness between the wild and farmed genotypes. ddRAD-Seq proved to be an effective method for detecting the discrete genetic structuring of C. oestroides and genotype intermixing between two populations. The parasite prevalence in the farmed sea bass was 1.02%, with a mean intensity of 2.0 and mean abundance of 0.02, while in the wild fish, the prevalence was 8.1%, the mean intensity, 1.81, and the mean abundance, 0.15. Such differences are likely a consequence of human interventions during the farmed fish's rearing cycle that, nevertheless, did not affect the transfer of C. oestroides.
Permanent Link: http://hdl.handle.net/11104/0328947