Occurrence and genetic diversity of Cryptosporidium spp. in wild foxes, wolves, jackals, and bears in central Europe

0554243 - BC 2022 RIV CZ eng J - Journal Article
Kváč, Martin - Myšková, E. - Holubová, Nikola - Kellnerová, K. - Kicia, M. - Rajský, D. - McEvoy, J. - Feng, Y. - Hanzal, V. - Sak, Bohumil
Occurrence and genetic diversity of Cryptosporidium spp. in wild foxes, wolves, jackals, and bears in central Europe.
Folia Parasitologica. Roč. 68, FEB 2 2021 (2021), č. článku 002. ISSN 0015-5683. E-ISSN 1803-6465
R&D Projects: GA MŠMT(CZ) LTAUSA17165
Institutional support: RVO:60077344
Keywords : n. apicomplexa cryptosporidiidae * molecular characterization * 1st report * red foxes * parasites * giardia * genotypes * mammals * parvum * ubiquitum * pcr * carnivores * genotyping * ssu * gp60 * microscopy * Czech Republic * Poland * Slovakia
OECD category: Veterinary science
Impact factor: 1.614, year: 2021
Method of publishing: Open access
https://folia.paru.cas.cz/artkey/fol-202101-0002_occurrence_and_genetic_diversity_of_cryptosporidium_spp_in_wild_foxes_wolves_jackals_and_bears_in_central_e.php

Parasites of the genus Cryptosporidium Tyzzer, 1910 are one of the most common protistan parasites of vertebrates. Faecal samples from 179 red foxes (Vulpes vulpes [Linnaeus]), 100 grey wolves (Canis lupus Linnaeus), 11 golden jackals (Canis aureus Linnaeus), and 63 brown bears (Ursus arctos Linnaeus) were collected in the Czech Republic, Poland and Slovakia. Samples were examined for the presence of Cryptosporidium spp. using microscopy and PCR/sequence analysis. Phylogenetic analysis based on the small subunit ribosomal RNA (SSU), actin and 60-kDa glycoprotein (gp60) genes using the maximum likelihood method revealed the presence of Cryptosporidium tyzzeri Ren, Zhao, Zhang, Ning, Jian et al., 2012 (n = 1) and C. andersoni Lindsay, Upton, Owens, Morgan, Mead et Blackburn, 2000 (n = 2) in red foxes, C. canis Fayer, Trout, Xiao, Morgan, Lai et Dubey, 2001 (n = 2) and C. ubiquitum Fayer, Santin et Macarisin, 2010 (n = 2) in grey wolves, and C. galli Pavlasek, 1999 in brown bears (n = 1) and red foxes (n = 1). Subtyping of isolates of C. ubiquitum and C. tyzzeri based on sequence analysis of gp60 showed that they belong to the XIId and IXa families, respectively. The presence of specific DNA of C. tyzzeri, C. andersoni and C. galli, which primarily infect the prey of carnivores, is probably the result of their passage through the gastrointestinal tract of the carnivores. Finding C. ubiquitum XIId in wolves may mean broadening the host spectrum of this subtype, but it remains possible this is the result of infected prey passing through the wolf in this case deer, which is a common host of this parasite. The dog genotype of C. canis was reported for the first time in wolves.
Permanent Link: http://hdl.handle.net/11104/0328869