Impact of Structural, Photochemical and Instrumental Effects on Leaf and Canopy Reflectance Variability in the 500-600 nm Range

0553571 - ÚVGZ 2023 RIV CH eng J - Journal Article
Moncholi-Estornell, A. - Van Wittenberghe, S. - Cendrero-Mateo, M. - Alonso, L. - Malenovský, Zbyněk - Moreno, J.
Impact of Structural, Photochemical and Instrumental Effects on Leaf and Canopy Reflectance Variability in the 500-600 nm Range.
Remote Sensing. Roč. 14, č. 1 (2022), č. článku 56. E-ISSN 2072-4292
Institutional support: RVO:86652079
Keywords : induced chlorophyll fluorescence * light-use efficiency * index pri * sun * photosynthesis * irradiance * quality * field * acclimation * temperature * proximal sensing * spectroscopy * protocols * irradiance * reflectance * vegetation index * sun- * shade-adapted leaves * xanthophyll cycle
OECD category: Climatic research
Impact factor: 5, year: 2022
Method of publishing: Open access
https://www.mdpi.com/2072-4292/14/1/56

Current rapid technological improvement in optical radiometric instrumentation provides an opportunity to develop innovative measurements protocols where the remote quantification of the plant physiological status can be determined with higher accuracy. In this study, the leaf and canopy reflectance variability in the PRI spectral region (i.e., 500-600 nm) is quantified using different laboratory protocols that consider both instrumental and experimental set-up aspects, as well as canopy structural effects and vegetation photoprotection dynamics. First, we studied how an incorrect characterization of the at-target incoming radiance translated into an erroneous vegetation reflectance spectrum and consequently in an incorrect quantification of reflectance indices such as PRI. The erroneous characterization of the at-target incoming radiance translated into a 2% overestimation and a 31% underestimation of estimated chlorophyll content and PRI-related vegetation indexes, respectively. Second, we investigated the dynamic xanthophyll pool and intrinsic Chl vs. Car long-term pool changes affecting the entire 500-600 nm spectral region. Consistent spectral behaviors were observed for leaf and canopy experiments. Sun-adapted plants showed a larger optical change in the PRI range and a higher capacity for photoprotection during the light transient time when compared to shade-adapted plants. Outcomes of this work highlight the importance of well-established spectroscopy sampling protocols to detect the subtle photochemical features which need to be disentangled from the structural and biological effects.
Permanent Link: http://hdl.handle.net/11104/0330967