Comparative Study Highlights the Potential of Spectral Deconvolution for Fucoxanthin Screening in Live Phaeodactylum tricornutum Cultures

0553567 - ÚVGZ 2023 RIV CH eng J - Journal Article
Macdonald Miller, S. - Abbriano, R. - Segečová, Anna - Herdean, A. - Ralph, P. J. - Pernice, M.
Comparative Study Highlights the Potential of Spectral Deconvolution for Fucoxanthin Screening in Live Phaeodactylum tricornutum Cultures.
Marine Drugs. Roč. 20, č. 1 (2022), č. článku 19. E-ISSN 1660-3397
Institutional support: RVO:86652079
Keywords : chlorophylls * quantification * fucoxanthin * microalgae * green consumption * food consumption
OECD category: Microbiology
Impact factor: 5.4, year: 2022
Method of publishing: Open access
https://www.mdpi.com/1660-3397/20/1/19

Microalgal biotechnology shows considerable promise as a sustainable contributor to a broad range of industrial avenues. The field is however limited by processing methods that have commonly hindered the progress of high throughput screening, and consequently development of improved microalgal strains. We tested various microplate reader and flow cytometer methods for monitoring the commercially relevant pigment fucoxanthin in the marine diatom Phaeodactylum tricornutum. Based on accuracy and flexibility, we chose one described previously to adapt to live culture samples using a microplate reader and achieved a high correlation to HPLC (R-2 = 0.849), effectively removing the need for solvent extraction. This was achieved by using new absorbance spectra inputs, reducing the detectable pigment library and changing pathlength values for the spectral deconvolution method in microplate reader format. Adaptation to 384-well microplates and removal of the need to equalize cultures by density further increased the screening rate. This work is of primary interest to projects requiring detection of biological pigments, and could theoretically be extended to other organisms and pigments of interest, improving the viability of microalgae biotechnology as a contributor to sustainable industry.
Permanent Link: http://hdl.handle.net/11104/0330990