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Size and Fluorescence Properties of Algal Photosynthetic Antenna Proteins Estimated by Microscopy

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    0553484 - MBÚ 2023 RIV CH eng J - Journal Article
    Crepin, Aurelia - Belgio, Erica - Šedivá, Barbora - Trsková, Eliška - Cunill-Semanat, Edel - Kaňa, Radek
    Size and Fluorescence Properties of Algal Photosynthetic Antenna Proteins Estimated by Microscopy.
    International Journal of Molecular Sciences. Roč. 23, č. 2 (2022), č. článku 778. E-ISSN 1422-0067
    R&D Projects: GA ČR(CZ) GA19-11494S; GA MŠMT(CZ) LO1416; GA MŠMT(CZ) ED2.1.00/19.0392
    Institutional support: RVO:61388971
    Keywords : light-harvesting-complex * photosystem-ii * chromera-velia * membrane * supercomplex * acclimation * plants * photosynthesis * antenna proteins * light-harvesting * fluorescence correlation spectroscopy * protein diffusion * microscopy * protein oligomerization * Chromera velia
    OECD category: Biochemistry and molecular biology
    Impact factor: 5.6, year: 2022
    Method of publishing: Open access
    https://www.mdpi.com/1422-0067/23/2/778

    Antenna proteins play a major role in the regulation of light-harvesting in photosynthesis. However, less is known about a possible link between their sizes (oligomerization state) and fluorescence intensity (number of photons emitted). Here, we used a microscopy-based method, Fluorescence Correlation Spectroscopy (FCS), to analyze different antenna proteins at the particle level. The direct comparison indicated that Chromera Light Harvesting (CLH) antenna particles (isolated from Chromera velia) behaved as the monomeric Light Harvesting Complex II (LHCII) (from higher plants), in terms of their radius (based on the diffusion time) and fluorescence yields. FCS data thus indicated a monomeric oligomerization state of algal CLH antenna (at our experimental conditions) that was later confirmed also by biochemical experiments. Additionally, our data provide a proof of concept that the FCS method is well suited to measure proteins sizes (oligomerization state) and fluorescence intensities (photon counts) of antenna proteins per single particle (monomers and oligomers). We proved that antenna monomers (CLH and LHCIIm) are more ´quenched´ than the corresponding trimers. The FCS measurement thus represents a useful experimental approach that allows studying the role of antenna oligomerization in the mechanism of photoprotection.
    Permanent Link: http://hdl.handle.net/11104/0331443

     
     
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