Non-cell autonomous and spatiotemporal signalling from a tissue organizer orchestrates root vascular development

0553392 - ÚEB 2022 RIV GB eng J - Journal Article
Yang, B. J. - Minne, M. - Brunoni, Federica - Plačková, Lenka - Petřík, Ivan - Sun, Y. - Nolf, J. - Smet, W. - Verstaen, K. - Wendrich, J.R. - Eekhout, T. - Hoyerová, Klára - Van Isterdael, G. - Haustraete, J. - Bishopp, A. - Farcot, E. - Novák, Ondřej - Saeys, Y. - De Rybel, B.
Non-cell autonomous and spatiotemporal signalling from a tissue organizer orchestrates root vascular development.
Nature Plants. Roč. 7, č. 11 (2021), s. 1485-1494. ISSN 2055-026X. E-ISSN 2055-0278
R&D Projects: GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : ARABIDOPSIS ROOT * BETA-GLUCOSIDASE * CYTOKININ ACTION
OECD category: Biochemistry and molecular biology
Impact factor: 17.352, year: 2021
Method of publishing: Open access
http://doi.org/10.1038/s41477-021-01017-6

During plant development, a precise balance of cytokinin is crucial for correct growth and patterning, but it remains unclear how this is achieved across different cell types and in the context of a growing organ. Here we show that in the root apical meristem, the TMO5/LHW complex increases active cytokinin levels via two cooperatively acting enzymes. By profiling the transcriptomic changes of increased cytokinin at single-cell level, we further show that this effect is counteracted by a tissue-specific increase in CYTOKININ OXIDASE 3 expression via direct activation of the mobile transcription factor SHORTROOT. In summary, we show that within the root meristem, xylem cells act as a local organizer of vascular development by non-autonomously regulating cytokinin levels in neighbouring procambium cells via sequential induction and repression modules.
Permanent Link: http://hdl.handle.net/11104/0328343