Xeno-Hybrid Bone Graft Releasing Biomimetic Proteins Promotes Osteogenic Differentiation of hMSCs

0553281 - ÚEM 2022 RIV CH eng J - Journal Article
Zhu, H. - Hefka Blahnová, Veronika - Perale, G. - Xiao, J. - Betge, F. - Boniolo, F. - Filová, Eva - Lyngstadaas, S. P. - Haugen, A.
Xeno-Hybrid Bone Graft Releasing Biomimetic Proteins Promotes Osteogenic Differentiation of hMSCs.
Frontiers in Cell and Developmental Biology. Roč. 8, dec. (2020), č. článku 619111. ISSN 2296-634X. E-ISSN 2296-634X
Institutional support: RVO:68378041
Keywords : bone scaffold * bone graft * bone regeneration biomimetic * bioactive proteins * intrinsically disordered * mesenchymal stem cells * xenograft
OECD category: Biomaterials (as related to medical implants, devices, sensors)
Impact factor: 6.684, year: 2020
Method of publishing: Open access
https://www.frontiersin.org/articles/10.3389/fcell.2020.619111/full

Bone defect is a noteworthy health problem and is the second most transplanted tissue after blood. Numerous bone grafts are designed and applied in clinics. Limitations, however, from different aspects still exist, including limited supply, mechanical strength, and bioactivity. In this study, two biomimetic peptides (P2 and P6) are incorporated into a composite bioactive xeno hybrid bone graft named SmartBonePep(R), with the aim to increase the bioactivity of the bone graft. The results, which include cytotoxicity, proliferation rate, confocal microscopy, gene expression, and protein qualification, successfully prove that the SmartBonePep(R) has multi-modal biological effects on human mesenchymal stem cells from bone marrow. The effective physical entrapment of P6 into a composite xeno-hybrid bone graft, withstanding manufacturing processes including exposure to strong organic solvents and ethylene oxide sterilization, increases the osteogenic potential of the stem cells as well as cell attachment and proliferation. P2 and P6 both show a strong biological potential and may be future candidates for enhancing the clinical performance of bone grafts.
Permanent Link: http://hdl.handle.net/11104/0328264