Cellular Response to Individual Components of the Platelet Concentrate

0553279 - ÚEM 2022 RIV CH eng J - Journal Article
Sovková, Věra - Vocetková, Karolína - Hedvičáková, Věra - Hefka Blahnová, Veronika - Buzgo, M. - Amler, E. - Filová, Eva
Cellular Response to Individual Components of the Platelet Concentrate.
International Journal of Molecular Sciences. Roč. 22, č. 9 (2021), č. článku 4539. E-ISSN 1422-0067
R&D Projects: GA MŠMT(CZ) LO1508
Institutional support: RVO:68378041
Keywords : platelets * plasma * mesenchymal stem cells * fibroblasts
OECD category: Cell biology
Impact factor: 6.208, year: 2021
Method of publishing: Open access
https://www.mdpi.com/1422-0067/22/9/4539

Platelet concentrates and especially their further product platelet lysate, are widely used as a replacement for cell culturing. Platelets contain a broad spectrum of growth factors and bioactive molecules that affect cellular fate. However, the cellular response to individual components of the human platelet concentrate is still unclear. The aim of this study was to observe cellular behavior according to the individual components of platelet concentrates. The bioactive molecule content was determined. The cells were supplemented with a medium containing 8% (v/v) of platelet proteins in plasma, pure platelet proteins in deionized water, and pure plasma. The results showed a higher concentration of fibrinogen, albumin, insulin growth factor I (IGF-1), keratinocyte growth factor (KGF), and hepatocyte growth factor (HGF), in the groups containing plasma. On the other hand, chemokine RANTES and platelet-derived growth factor bb (PDGF-bb), were higher in the groups containing platelet proteins. The groups containing both plasma and plasma proteins showed the most pronounced proliferation and viability of mesenchymal stem cells and fibroblasts. The platelet proteins alone were not sufficient to provide optimal cell growth and viability. A synergic effect of platelet proteins and plasma was observed. The data indicated the importance of plasma in platelet lysate for cell growth
Permanent Link: http://hdl.handle.net/11104/0328262