Glyphosate Interaction with eEF1 alpha 1 Indicates Altered Protein Synthesis: Evidence for Reduced Spermatogenesis and Cytostatic Effect

0553278 - ÚEM 2022 RIV US eng J - Journal Article
Sopko, Bruno - Tejral, Gracian - Bitti, Giuseppe - Abate, M. - Medvedíková, M. - Hajduch, M. - Chloupek, J. - Fajmonová, J. - Skorič, M. - Amler, E. - Erban, T.
Glyphosate Interaction with eEF1 alpha 1 Indicates Altered Protein Synthesis: Evidence for Reduced Spermatogenesis and Cytostatic Effect.
ACS Omega. Roč. 6, č. 23 (2021), s. 14848-14857. ISSN 2470-1343. E-ISSN 2470-1343
Institutional support: RVO:68378041
Keywords : transfer-RNA synthetases * non-hodgkin-lymphoma * estrogen-receptor * cell-growth * exposure * expression
OECD category: Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions (pharmacogenomics, gene-based therapeutics)
Impact factor: 4.132, year: 2021
Method of publishing: Open access
https://pubs.acs.org/doi/10.1021/acsomega.1c00449

The broad-spectrum herbicide, glyphosate, is considered safe for animals because it selectively affects the shikimate pathway that is specific to plants and microorganisms. We sought a previously unknown mechanism to explain the concerns that glyphosate exposure can negatively affect animals, including humans. Computer modeling showed a probable interaction between glyphosate and eukaryotic translation elongation factor 1 subunit alpha 1 (eEF1 alpha 1), which was confirmed by microcalorimetry. Only restricted, nondisrupted spermatogenesis in rats was observed after chronic glyphosate treatments (0.7 and 7 mg/L). Cytostatic and antiproliferative effects of glyphosate in GC-1 and SUP-B15 cells were indicated. Meta-analysis of public health data suggested a possible effect of glyphosate use on sperm count. The in silico, in vitro, and in vivo experimental results as well as the metastatistics indicate side effects of chronic glyphosate exposure. Together, these findings indicate that glyphosate delays protein synthesis through an interaction with eEF1 alpha 1, thereby suppressing spermatogenesis and cell growth.
Permanent Link: http://hdl.handle.net/11104/0328261