The distinctive flagellar proteome of Euglena gracilis illuminates the complexities of protistan flagella adaptation

0553213 - BC 2022 RIV GB eng J - Journal Article
Hammond, Michael John - Zoltner, M. - Garrigan, J. - Butterfield, E. - Varga, Vladimír - Lukeš, Julius - Field, Mark Christian
The distinctive flagellar proteome of Euglena gracilis illuminates the complexities of protistan flagella adaptation.
New Phytologist. Roč. 232, č. 3 (2021), s. 1323-1336. ISSN 0028-646X. E-ISSN 1469-8137
R&D Projects: GA MŠMT(CZ) EF16_019/0000759; GA MŠMT(CZ) LL1601; GA ČR(CZ) GA18-15962S
EU Projects: Wellcome Trust(GB) 204697/Z/16/Z
Grant - others:Medical Research Council UK (MRC)(GB) MR/P009018/1
Institutional support: RVO:60077344 ; RVO:68378050
Keywords : adenylyl-cyclase pac * signal-transduction * proteins * transport * genome * rod * localization * components * diversity * phylogeny * cilia * Euglena * evolution * flagella * pellicle * proteomics
OECD category: Genetics and heredity (medical genetics to be 3); Genetics and heredity (medical genetics to be 3) (UMG-J)
Impact factor: 10.323, year: 2021
Method of publishing: Open access
https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.17638

The eukaryotic flagellum/cilium is a prominent organelle with conserved structure and diverse functions. Euglena gracilis, a photosynthetic and highly adaptable protist, employs its flagella for both locomotion and environmental sensing. Using proteomics of isolated E. gracilis flagella we identify nearly 1700 protein groups, which challenges previous estimates of the protein complexity of motile eukaryotic flagella. We not only identified several unexpected similarities shared with mammalian flagella, including an entire glycolytic pathway and proteasome, but also document a vast array of flagella-based signal transduction components that coordinate gravitaxis and phototactic motility. By contrast, the pellicle was found to consist of > 900 protein groups, containing additional structural and signalling components. Our data identify significant adaptations within the E. gracilis flagellum, many of which are clearly linked to the highly flexible lifestyle.
Permanent Link: http://hdl.handle.net/11104/0328222