Functionalized terpolymer-brush-based biointerface with improved antifouling properties for ultra-sensitive direct detection of virus in crude clinical samples

0552755 - FZÚ 2022 RIV US eng J - Journal Article
Forinová, Michala - Pilipenco, Alina - Víšová, Ivana - Lynn, Nicholas Scott - Dostálek, Jakub - Mašková, H. - Hönig, Václav - Palus, Martin - Selinger, Martin - Kočová, P. - Dyčka, F. - Štěrba, J. - Houska, Milan - Vrabcová, Markéta - Horák, Petr - Anthi, Judita - Tung, C.P. - Yu, C.M. - Chen, C.Y. - Huang, Y.C. - Tsai, P.H. - Lin, S.Y. - Hsu, H.J. - Yang, A.S. - Dejneka, Alexandr - Vaisocherová-Lísalová, Hana
Functionalized terpolymer-brush-based biointerface with improved antifouling properties for ultra-sensitive direct detection of virus in crude clinical samples.
ACS Applied Materials and Interfaces. Roč. 13, č. 50 (2021), s. 60612-60624. ISSN 1944-8244. E-ISSN 1944-8252
R&D Projects: GA MŠMT(CZ) EF18_053/0016627; GA ČR(CZ) GA21-19779S
Grant - others:AV ČR LQ100101902; OP VVV - Mobility FZU 2(XE) CZ.02.2.69/0.0/0.0/18_053/0016627
Program: Prémie Lumina quaeruntur
Institutional support: RVO:68378271 ; RVO:60077344
Keywords : functional coatings * polymer brush * zwitterionic materials * SARS-CoV-2 * piezoelectric biosensor * rapid detection
OECD category: Biochemical research methods; Infectious Diseases (BC-A)
Impact factor: 10.383, year: 2021
Method of publishing: Limited access
https://doi.org/10.1021/acsami.1c16930

New analytical techniques that overcome major drawbacks of current routinely used viral infection diagnosis methods, i.e., the long analysis time and laboriousness of real-time reversetranscription polymerase chain reaction (qRT-PCR) and the insufficient sensitivity of “antigen tests”, are urgently needed in the context of SARS-CoV-2 and other highly contagious viruses. Here, we report on an antifouling terpolymer-brush biointerface that enables the rapid and sensitive detection of SARSCoV-2 in untreated clinical samples. The developed biointerface carries a tailored composition of zwitterionic and non-ionic moieties and allows for the significant improvement of antifouling capabilities when postmodified with biorecognition elements and exposed to complex media.

Permanent Link: http://hdl.handle.net/11104/0327875