Post-embryonic lateral organ development and adaxial–abaxial polarity are regulated by the combined effect of enhancer of shoot regeneration 1 and wuschel in arabidopsis shoots

0552742 - ÚEB 2022 RIV CH eng J - Journal Article
Ikeda, Y. - Králová, M. - Zalabák, David - Kubalová, I. - Aida, M.
Post-embryonic lateral organ development and adaxial–abaxial polarity are regulated by the combined effect of enhancer of shoot regeneration 1 and wuschel in arabidopsis shoots.
International Journal of Molecular Sciences. Roč. 22, č. 19 (2021), č. článku 10621. E-ISSN 1422-0067
R&D Projects: GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : Adaxial–abaxial polarity * Arabidopsis thaliana * enhancer of shoot regeneration 1 * Lateral organ * revolta * Shoot apical meristem * shootmeristemless * wuschel
OECD category: Plant sciences, botany
Impact factor: 6.208, year: 2021
Method of publishing: Open access
http://doi.org/10.3390/ijms221910621

The development of above-ground lateral organs is initiated at the peripheral zone of the shoot apical meristem (SAM). The coordination of cell fate determination and the maintenance of stem cells are achieved through a complex regulatory network comprised of transcription factors. Two AP2/ERF transcription factor family genes, ESR1/DRN and ESR2/DRNL/SOB/BOL, regulate cotyledon and flower formation and de novo organogenesis in tissue culture. However, their roles in post-embryonic lateral organ development remain elusive. In this study, we analyzed the genetic interactions among SAM-related genes, WUS and STM, two ESR genes, and one of the HD-ZIP III members, REV, whose protein product interacts with ESR1 in planta. We found that esr1 mutations substantially enhanced the wus and stm phenotypes, which bear a striking resemblance to those of the wus rev and stm rev double mutants, respectively. Aberrant adaxial–abaxial polarity is observed in wus esr1 at relatively low penetrance. On the contrary, the esr2 mutation partially suppressed stm phenotypes in the later vegetative phase. Such complex genetic interactions appear to be attributed to the distinct expression pattern of two ESR genes because the ESR1 promoter-driving ESR2 is capable of rescuing phenotypes caused by the esr1 mutation. Our results pose the unique genetic relevance of ESR1 and the SAM-related gene interactions in the development of rosette leaves.
Permanent Link: http://hdl.handle.net/11104/0327858