Cytological and Molecular Characterization for Ploidy Determination in Yams (Dioscorea spp.)

0552702 - ÚEB 2022 RIV CH eng J - Journal Article
Gatarira, C. - Sládeková, Lucia - Němečková, Alžběta - Šimoníková, Denisa - Paliwal, R. - Asfaw, A. - Abberton, M. - Badara, G. - Asiedu, R. - Čížková, Jana - Hřibová, Eva - Doležel, Jaroslav - DeKoeyer, D. - Adetimirin, V. - Bhattacharjee, R.
Cytological and Molecular Characterization for Ploidy Determination in Yams (Dioscorea spp.).
Agronomy. Roč. 11, č. 10 (2021), č. článku 1897. E-ISSN 2073-4395
R&D Projects: GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : nuclear-DNA content * flow-cytometry * genetic diversity * alata l. * plants * microsatellite * musa * relatedness * association * polyploidy * flow cytometry * chromosome counting * ploidy level * DArTseq
OECD category: Biochemistry and molecular biology
Impact factor: 3.949, year: 2021
Method of publishing: Open access
http://doi.org/10.3390/agronomy11101897

Yam (Dioscorea spp.) is a monocotyledonous herbaceous vine plant grown in the tropics and subtropics. It is a multi-species plant with varied intra- and interspecific ploidy levels. Of the 600 species, 11 are cultivated supporting the livelihood of over 300 million people. The paucity of information on ploidy and the genomic constitution is a significant challenge to the crop's genetic improvement through crossbreeding. The objective of this study was to investigate the ploidy levels of 236 accessions across six cultivated and two wild species using chromosome counting, flow cytometry and genotyping-based ploidy determination methods. Results obtained from chromosome counting and genotyping-based ploidy determination were in agreement. In majority of the accessions, chromosome counting and flow cytometry were congruent, allowing future rapid screening of ploidy levels using flow cytometry. Among cultivated accessions, 168 (71%) were diploid, 50 (21%) were triploid, and 12 (5%) were tetraploid. Two wild species included in the study were diploids. Resolution of ploidy level in yams offers opportunities for implementing successful breeding programmes through intra- and interspecific hybridization.
Permanent Link: http://hdl.handle.net/11104/0327828