Sensitive identification of milk protein allergens using on-line combination of transient isotachophoresis/micellar electrokinetic chromatography and capillary isoelectric focusing in fused silica capillary with roughened part

0552182 - ÚIACH 2023 RIV NL eng J - Journal Article
Horká, Marie - Šalplachta, Jiří - Karásek, Pavel - Roth, Michal
Sensitive identification of milk protein allergens using on-line combination of transient isotachophoresis/micellar electrokinetic chromatography and capillary isoelectric focusing in fused silica capillary with roughened part.
Food Chemistry. Roč. 377, MAY (2022), č. článku 131986. ISSN 0308-8146. E-ISSN 1873-7072
R&D Projects: GA ČR(CZ) GA19-00742S; GA MV(CZ) VI04000062
Institutional support: RVO:68081715
Keywords : capillary electrophoretic methods * adhesion of milk protein allergens
OECD category: Analytical chemistry
Impact factor: 8.8, year: 2022
Method of publishing: Limited access

This study describes a method for on-line concentration of milk proteins from large sample volumes using combination of transient isotachophoresis (tITP) and micellar electrokinetic chromatography (MEKC) in fused silica capillary with an inner roughened part. The tITP/MEKC method was further combined with capillary isoelectric focusing (CIEF) for on-line concentration, separation, identification and sensitive determination of proteins in skimmed milk.
Permanent Link: http://hdl.handle.net/11104/0327320