Lysosome dysfunction induced by magnetic nanoparticles: is it an answer how to regulate the cell death remotely?

0552010 - FZÚ 2022 NL eng A - Abstract
Uzhytchak, Mariia - Smolková, Barbora - Lunova, Mariia - Frtús, Adam - Dejneka, Alexandr - Lunov, Oleg
Lysosome dysfunction induced by magnetic nanoparticles: is it an answer how to regulate the cell death remotely?
FEBS Open Bio. Vol. 11. Amsterdam: Wiley, 2021. s. 257-257. ISSN 2211-5463.
[FEBS Congress, Molecules of Life: Towards New Horizons /45./. 03.07.2021-08.07.2021, online]
Institutional support: RVO:68378271
Keywords : magnetic nanoparticles * lysosomes * autophagy
OECD category: Biophysics
https://febs.onlinelibrary.wiley.com/doi/10.1002/2211-5463.13205

Magnetic nanoparticles (NP) became a cornerstone in modern biomedicine and cell biology as a promising tool for diagnostics, targeted drug delivery and bioimaging. We investigated cell lysosomalmediated signaling in three hepatic cell lines (Huh7, HepG2 and Alexander) over magnetic nanoparticles treatment in different concentrations. Nanoparticle treatment resulted in reorganization of Factin and tubulin cytoskeletal filaments. Lysosomal accumulation of nanoparticles induced significant lysosomal dysfunction and affected mTOR activity. Further, we found that nanoparticle treatment triggered autophagic flux in Alexander and Huh7 cells, whereas HepG2 remained unresponsive to the treatment. Indeed, Bcl2 protein expression level was a key to the resistance of HepG2 cells to nanoparticle treatment.
Permanent Link: http://hdl.handle.net/11104/0327197