Individual copy number variation and extensive diversity between major MHC-DAB1 allelic lineages in the European bitterling

0551782 - ÚBO 2023 RIV DE eng J - Journal Article
Talarico, L. - Bryjová, Anna - Čížková, Dagmar - Douda, K. - Reichard, Martin
Individual copy number variation and extensive diversity between major MHC-DAB1 allelic lineages in the European bitterling.
Immunogenetics. Roč. 74, č. 5 (2022), s. 497-505. ISSN 0093-7711. E-ISSN 1432-1211
R&D Projects: GA ČR GA19-05510S
Institutional support: RVO:68081766
Keywords : Rhodeus amarus * MHC class IIB * Positive selection * Gene duplication * MHC supertypes * Positively selected sites
OECD category: Marine biology, freshwater biology, limnology
Impact factor: 3.2, year: 2022
Method of publishing: Open access
https://link.springer.com/article/10.1007%2Fs00251-021-01251-4

Polymorphism of the major histocompatibility complex (MHC), DAB1 gene was characterized for the first time in the European bitterling (Rhodeus amarus), a freshwater fish employed in studies of host-parasite coevolution and mate choice, taking advantage of newly designed primers coupled with high-throughput amplicon sequencing. Across 221 genotyped individuals, we detected 1-4 variants per fish, with 28% individuals possessing 3-4 variants. We identified 36 DAB1 variants, and they showed high sequence diversity mostly located within predicted antigen-binding sites, and both global and codon-specific excess of non-synonymous mutations. Despite deep divergence between two major allelic lineages, functional diversity was surprisingly low (3 supertypes). Overall, these findings suggest the role of positive and balancing selection in promotion and long-time maintenance of DAB1 polymorphism. Further investigations will clarify the role of pathogen-mediated selection to drive the evolution of DAB1 variation.
Permanent Link: http://hdl.handle.net/11104/0326999