Targeted Sequencing of the Short Arm of Chromosome 6V of a Wheat Relative Haynaldia villosa for Marker Development and Gene Mining

0551743 - ÚEB 2022 RIV CH eng J - Journal Article
Zhang, X. - Wan, W. - Li, M. - Yu, Z. - Liu, J. - Holušová, Kateřina - Vrána, Jan - Doležel, Jaroslav - Wu, Y. - Wang, H. - Xiao, J. - Wang, X.
Targeted Sequencing of the Short Arm of Chromosome 6V of a Wheat Relative Haynaldia villosa for Marker Development and Gene Mining.
BASELSEP 2021: MDPI, 2021. Agronomy. Roč. 11, č. 9 (2021), č. článku 1695. E-ISSN 2073-4395
R&D Projects: GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : triticum-aestivum * dasypyrum-villosum * genome sequence * draft genome * identification * translocation * introgression * resistance * reveals * comparative genome analysis * chromosome sorting * genome sequencing * marker development * flow cytometry * Haynaldia villosa
OECD category: Biochemistry and molecular biology
Impact factor: 3.949, year: 2021
Method of publishing: Open access
http://doi.org/10.3390/agronomy11091695

The short arm of chromosome 6V (6VS) of Haynaldia villosa has been used in wheat breeding programs to introduce Pm21 resistance gene against powdery mildew (Pm) and some other genes. In this this study, 6VS was flow-sorted from wheat-H. villosa ditelosomic addition line Dt6VS and sequenced by Illumina technology. An assembly of 230.39 Mb was built with contig N50 of 9.788 bp. In total, 3.276 high-confidence genes were annotated and supported by RNA sequencing data. Repetitive elements represented 74.91% of the 6VS assembly. The 6VS homologous genes were identified on homologous group 6 in six Triticeae species confirming their synteny relationships. Out of 45 NB-ARC domain proteins identified on 6VS, 15 were upregulated and might also be involved in the innate immunity of H. villosa to Pm. High thousand grain weight (TGW) for 6VS/6AL translocation line was not attributable to GW2-6V gene. Based on the intron size differences, 119 intron-target (IT) markers were developed to trace the 6VS chromatins introduced into wheat background. The assembled 6VS genome sequence and the developed 6VS specific IT markers in this work will facilitate the gene mining and utilization of agronomic important genes on 6VS.
Permanent Link: http://hdl.handle.net/11104/0326965