Detecting attomolar concentrations of microRNA related to myelodysplastic syndromes in blood plasma using a novel sandwich assay with nanoparticle release

0551739 - ÚFE 2022 RIV NL eng J - Journal Article
Špringer, Tomáš - Krejčík, Z. - Homola, Jiří
Detecting attomolar concentrations of microRNA related to myelodysplastic syndromes in blood plasma using a novel sandwich assay with nanoparticle release.
Biosensors and Bioelectronics. Roč. 104, DEC 15 2021 (2021), č. článku 113613. ISSN 0956-5663. E-ISSN 1873-4235
R&D Projects: GA ČR(CZ) GX20-23787X
Institutional support: RVO:67985882
Keywords : Detection of miRNA * Optical affinity biosensor * Gold nanoparticles * Surface plasmon resonance biosensor
OECD category: Optics (including laser optics and quantum optics)
Impact factor: 12.545, year: 2021
Method of publishing: Open access
https://doi.org/10.1016/j.bios.2021.113613

Microribonucleic acids (miRNAs) are short noncoding ribonucleic acids that have been linked with a multitude of human diseases including lung, breast, and hematological cancers. In this work, we present a novel, extremely sensitive assay for the label-free optical biosensor-based detection of miRNAs, which is based on the oligonucleotide-triggered release of nanoparticles from a sensor surface. We combine this assay (herein referred to as the nanoparticle-release (NPR) assay) with a surface plasmon resonance biosensor and show that the assay is able to enhance the specific sensor response associated with the binding of target miRNA while suppressing the interfering effects caused by the non-specific binding. We apply the assay to the detection of miRNAs related to myelodysplastic syndromes (miR-125b, miR-16) in blood plasma and demonstrate that the assay enables detection of miR-125b with a limit of detection (LOD) of 349 aM (corresponding to the lowest detectable amounts of 419 zmol). The achieved LOD is better by a factor of -100 when compared to the conventional nanoparticle-enhanced sandwich assay. Moreover, we demonstrate that the NPR assay may be combined with time-division multiplexing for the multiplexed miRNA detection
Permanent Link: http://hdl.handle.net/11104/0326961