Lactylated acidic exopolysaccharide produced by the cyanobacterium Nostoc cf. linckia

0551541 - BÚ 2023 RIV GB eng J - Journal Article
Uhliariková, I. - Matulová, M. - Košťálová, Z. - Lukavský, Jaromír - Čapek, P.
Lactylated acidic exopolysaccharide produced by the cyanobacterium Nostoc cf. linckia.
Carbohydrate Polymers. Roč. 276, JAN 15 2022 (2022), č. článku 118801. ISSN 0144-8617. E-ISSN 1879-1344
R&D Projects: GA TA ČR TE01020080
Institutional support: RVO:67985939
Keywords : Nostoc. cf. linckyia * acidic exopolysacharides * structure * nosturonic acid:3-O-lactyl-β-D-glucuronic acid * antioxidant acitivity
OECD category: Cell biology
Impact factor: 11.2, year: 2022
Method of publishing: Limited access
https://doi.org/10.1016/j.carbpol.2021.118801

Cyanobacteria produce a wide range of metabolites of interest for industrial or medical use. The cultivation of freshwater Nostoc cf. linckia yielded 5.4 g/L of a crude exopolysaccharide (cEPS) with a molecular weight of 1.31 × 105 g/mol. Ion-exchange chromatography of cEPS yielded two dominant fractions, EPS-1 and EPS-2, differing in molecular weight. The lower molecular weight fraction (EPS-1) was subjected to structural studies. Results of chemical and spectroscopic analyses showed that three of the four dominant sugars, glucose, galactose and
xylose are 1,4-linked in the backbone in the following order: [→4)-β-D-Xylp-(1 → 4)-β-D-Glcp-(1 → 4)-α-D-Galp-(1 → 4)-β-D-Glcp-(1→]n. Terminal mannose residues were identified as side chains linked at C3 of every third backbone xylose and every second glucose is branched at C6 by 3-O-lactyl-β-D-glucuronic acid (nosturonic acid). Antioxidant properties of EPS were tested using two in vitro methods. Both assays showed that the cEPS was more active than purified EPS-1 and EPS-2 fractions and deproteinized EPS.
Permanent Link: http://hdl.handle.net/11104/0330527