Cationic electrophoretic focusing on inverse electromigration dispersion profile with ESI-MS detection. New capillary electrophoretic method for high-sensitivity analysis of 2-hydroxy-s-triazines in waters

0551190 - ÚIACH 2023 RIV NL eng J - Journal Article
Malá, Zdeňka - Gebauer, Petr
Cationic electrophoretic focusing on inverse electromigration dispersion profile with ESI-MS detection. New capillary electrophoretic method for high-sensitivity analysis of 2-hydroxy-s-triazines in waters.
Analytica Chimica Acta. Roč. 1195, FEB (2022), č. článku 339477. ISSN 0003-2670. E-ISSN 1873-4324
Institutional support: RVO:68081715
Keywords : capillary electrophoresis * electrophoretic focusing * ESI-MS detection
OECD category: Analytical chemistry
Impact factor: 6.2, year: 2022
Method of publishing: Limited access

Electrophoretic focusing on an inverse electromigration dispersion profile is a relatively new electrophoretic separation method for the analysis of ionisable analytes. Although it offers alternative selectivity, powerful concentration capability and high sensitivity, so far only several systems for the analysis of anionic compounds were reported. This work describes for the first time a functional electrolyte system for the separation of cationic analytes by this method. Based on computer simulations and calculations, a functional electrolyte system was designed, based on 1-ethylpiperazine and pivalic acid. It is suitable for the analysis of weak bases with pKa values between 4.5 and 6 and due to the volatility of both components, it can be operated in combination with ESI-MS detection. Its practical applicability is demonstrated on direct analysis of four 2-hydroxy-s-triazines in waters. Separation in an uncoated capillary with application of negative pressure at the inlet provides very satisfactory results with good reproducibility of peak areas, peak heights and detection times, and with LODs at the 0.5 -1 nM level. The robustness of the method is shown on analyses of real tap and river water samples used without any pretreatment.
Permanent Link: http://hdl.handle.net/11104/0326629