Mass Spectrometry Imaging of Small Molecules: Methods and Protocols

0551179 - ÚOCHB 2023 RIV US eng M - Monography Chapter
Strnad, Štěpán - Strnadová, Veronika - Sýkora, D. - Cvačka, Josef - Maletínská, Lenka - Vrkoslav, Vladimír
MALDI Mass Spectrometry Imaging of Lipids on Free-Floating Brain Sections and Immunohistochemically Colocalized Markers of Neurodegeneration.
Mass Spectrometry Imaging of Small Molecules: Methods and Protocols. New York: Humana Press, 2022 - (Lee, Y.), s. 229-239. Methods in Molecular Biology, 2437. ISBN 978-1-07162029-8
R&D Projects: GA ČR(CZ) GA20-00546S
Institutional support: RVO:61388963
Keywords : colocalization * free-floating * immunohistochemistry * MALDI MSI * multimodal * tissue section
OECD category: Physiology (including cytology)
https://doi.org/10.1007/978-1-0716-2030-4_16

In mass spectrometry imaging (MSI), the essential steps in sample preparation include collection and storage. The most widely used preservation procedure for MSI consists in freezing samples and storing them at temperatures below −80 °C. On the other hand, the most common method for preserving biological samples in clinical practice is their fixation in paraformaldehyde. The storage of free-floating sections is a particular type of the preservation of paraformaldehyde-fixed tissues that is used in immunohistochemistry. This chapter describes the approach of the multimodal imaging of free-floating brain sections using the MSI of lipids and the immunohistochemistry of neurodegeneration markers.
Permanent Link: http://hdl.handle.net/11104/0326619