Insoluble hyaluronan films obtained by heterogeneous crosslinking with iron(III) as resorbable implants

0551016 - BFÚ 2022 RIV GB eng J - Journal Article
Mrázek, J. - Kohout, J. - Kmjec, T. - Nešporová, K. - Chmelař, J. - Kubala, Lukáš - Velebný, V.
Insoluble hyaluronan films obtained by heterogeneous crosslinking with iron(III) as resorbable implants.
International Journal of Biological Macromolecules. Roč. 191, NOV 30 2021 (2021), s. 201-210. ISSN 0141-8130. E-ISSN 1879-0003
Institutional support: RVO:68081707
Keywords : acid hydrogel films * complexes * prevention * adhesions * scaffolds
OECD category: Biochemistry and molecular biology
Impact factor: 8.025, year: 2021
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0141813021019802?via%3Dihub

We present water-insoluble hyaluronan films crosslinked by trivalent iron developed as potential resorbable implants. The films were crosslinked by sorption of ferric salt into solid HA films in water/2-propanol bath. These heterogeneously crosslinked films (het-FeHA) remained tough and dimensionally stable when rehydrated in saline. In contrast, films prepared by drying the well-known homogeneous ferric hyaluronate gels (hom-FeHA) softened upon rehydration and expanded rapidly. Differences between hom-FeHA and het-FeHA result from polymer network topology (dominant inter-or intra-molecular crosslink, respectively). Moreover, Mossbauer spectroscopy of het-FeHA revealed diiron complexes, while iron in the hom-FeHA was present exclusively as gamma-FeOOH nanoparticles or amorphous FeOOH. The biocompatibility tests of het-FeHA did not show any adverse effect and the sample disintegrated within one day when implanted in mice peritoneum. In conclusion, we developed implantable hyaluronan-based free-standing film with minimal swelling that can be resorbed quickly enough to avoid induction of foreign-body reaction.
Permanent Link: http://hdl.handle.net/11104/0326300