Quantitative analysis of neuronal mitochondrial movement reveals patterns resulting from neurotoxicity of rotenone and 6-hydroxydopamine

0550586 - BTÚ 2022 RIV US eng J - Journal Article
Simões, R. F. - Pino, R. - Moreira-Soares, M. - Kovářová, Jaromíra - Neužil, Jiří - Travasso, R. - Oliveira, P. J. - Cunha-Oliveira, T. - Pereira, F. B.
Quantitative analysis of neuronal mitochondrial movement reveals patterns resulting from neurotoxicity of rotenone and 6-hydroxydopamine.
FASEB Journal. Roč. 35, č. 12 (2021), č. článku e22024. ISSN 0892-6638. E-ISSN 1530-6860
R&D Projects: GA ČR(CZ) GA20-05942S; GA ČR(CZ) GX21-04607X
Institutional support: RVO:86652036
Keywords : axonal-transport * parkinsons-disease * oxidative damage * animal-models
OECD category: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Impact factor: 5.834, year: 2021
Method of publishing: Open access
https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202100899R

Alterations in mitochondrial dynamics, including their intracellular trafficking, are common early manifestations of neuronal degeneration. However, current methodologies used to study mitochondrial trafficking events rely on parameters that are primarily altered in later stages of neurodegeneration. Our objective was to establish a reliable applied statistical analysis to detect early alterations in neuronal mitochondrial trafficking. We propose a novel quantitative analysis of mitochondria trajectories based on innovative movement descriptors, including straightness, efficiency, anisotropy, and kurtosis. We evaluated time- and dose-dependent alterations in trajectory descriptors using biological data from differentiated SH-SY5Y cells treated with the mitochondrial toxicants 6-hydroxydopamine and rotenone. MitoTracker Red CMXRos-labelled mitochondria movement was analyzed by total internal reflection fluorescence microscopy followed by computational modelling to describe the process. Based on the aforementioned trajectory descriptors, this innovative analysis of mitochondria trajectories provides insights into mitochondrial movement characteristics and can be a consistent and sensitive method to detect alterations in mitochondrial trafficking occurring in the earliest time points of neurodegeneration.
Permanent Link: http://hdl.handle.net/11104/0328033