Strigolactones

0550561 - BTÚ 2022 RIV US eng M - Monography Chapter
Andersson, Inger - Carlsson, G. H. - Hasse, D.
Structural Analysis of Strigolactone-Related Gene Products.
Methods in Molecular Biology. In: Strigolactones. Berlin: Springer Science+Business Media, 2021 - (Prandi, C.; Cardinale, F.), s. 245-257. ISBN 978-1-0716-1429-7
R&D Projects: GA MŠMT EF15_003/0000447
Institutional support: RVO:86652036
Keywords : Strigolactone * Strigolactone receptors * Macromolecular crystallography
OECD category: Biochemistry and molecular biology
Method of publishing: Limited access
https://link.springer.com/protocol/10.1007/978-1-0716-1429-7_19

Structural knowledge of biological macromolecules is essential for understanding their function and for modifying that function by engineering. Protein crystallography is a powerful method for elucidating molecular structures of proteins, but it is essential that the investigator has a basic knowledge of good practices and of the major pitfalls in the technique. Here we describe issues specific for the case of structural studies of strigolactone (SL) receptor structure and function, and in particular the difficulties associated with capturing complexes of SL receptors with the SL hormone ligand in the crystal.
Permanent Link: http://hdl.handle.net/11104/0329287