Human “barcode”: Link between phosphate intensity changes in human enamel and light microscopy record of accentuated lines

0550243 - ARÚB 2022 RIV US eng J - Journal Article
Vacková, Soňa - Králik, M. - Marečková, K. - Ráčková, L. - Quade, L. - Sedláčková, L. - Fojtík, P. - Kučera, L.
Human “barcode”: Link between phosphate intensity changes in human enamel and light microscopy record of accentuated lines.
Microchemical Journal. Roč. 168, September 2021 (2021), č. článku 106370. ISSN 0026-265X. E-ISSN 1095-9149
Institutional support: RVO:68081758
Keywords : dental development * raman microscopy * transmitted light microscopy * thin section * dental enamel * phosphate * accentuated stress lines
OECD category: Archaeology
Impact factor: 5.304, year: 2021
Method of publishing: Open access
https://doi.org/10.1016/j.microc.2021.106370

Disruption of metabolic processes during human enamel mineralization may result in the appearance of accentuated lines in enamel. These accentuated lines in the enamel relate to stress events that were experienced and overcome in early ontogenetic phases. Accentuated lines are visible in transmitted light microscopy as dark areas. However, their appearance is variable even within single tooth. The objective of this study is to analyze the distribution of phosphate in human enamel with a focus on accentuated stress lines by Raman microscopy. Transmitted light microscopy and Raman microscopy records of 15 human teeth (11 samples from archaeological excavations, 4 from recent population) were compared, using visual evaluation and statistical methods (correlation of pixel light intensities in the microscopic images). The data obtained by Raman microscopy suggests a relationship between low phosphate content (signal at 960 cm- 1) and accentuated stress lines in human enamel.
Permanent Link: http://hdl.handle.net/11104/0330755