Quantifying Cyanothece growth under DIC limitation

0550181 - MBÚ 2022 RIV NL eng J - Journal Article
Inomura, K. - Masuda, Takako - Eichner, Meri - Rabouille, S. - Zavřel, Tomáš - Červený, Jan - Vancová, Marie - Bernát, Gábor - Armin, G. - Claquin, P. - Kotabová, Eva - Stephan, S. - Suggett, D.J. - Deutsch, C. - Prášil, Ondřej
Quantifying Cyanothece growth under DIC limitation.
Computational and Structural Biotechnology Journal. Roč. 19, č. 2021 (2021), s. 6456-6464. ISSN 2001-0370. E-ISSN 2001-0370
R&D Projects: GA ČR(CZ) GA20-17627S; GA ČR(CZ) GA18-24397S; GA MŠMT(CZ) LM2018129
Research Infrastructure: Czech-BioImaging II - 90129
Institutional support: RVO:61388971 ; RVO:86652079 ; RVO:60077344
Keywords : Cyanothece * DIC * CO2 * Nitrogen fixation * Nitrate * Diurnal Cyanothece * Carbon * Photosynthesis * Quantitative model
OECD category: Microbiology; Microbiology (UEK-B); Cell biology (BC-A)
Impact factor: 6.155, year: 2021
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S2001037021005018?via%3Dihub

The photoautotrophic, unicellular N-2-fixer, Cyanothece, is a model organism that has been widely used to study photosynthesis regulation, the structure of photosystems, and the temporal segregation of carbon (C) and nitrogen (N) fixation in light and dark phases of the diel cycle. Here, we present a simple quantitative model and experimental data that together, suggest external dissolved inorganic carbon (DIC) concentration as a major limiting factor for Cyanothece growth, due to its high C-storage requirement. Using experimental data from a parallel laboratory study as a basis, we show that after the onset of the light period, DIC was rapidly consumed by photosynthesis, leading to a sharp drop in the rate of photosynthesis and C accumulation. In N-2-fixing cultures, high rates of photosynthesis in the morning enabled rapid conversion of DIC to intracellular C storage, hastening DIC consumption to levels that limited further uptake. The N-2-fixing condition allows only a small fraction of fixed C for cellular growth since a large fraction was reserved in storage to fuel night-time N-2 fixation. Our model provides a framework for resolving DIC limitation in aquatic ecosystem simulations, where DIC as a growth-limiting factor has rarely been considered, and importantly emphasizes the effect of intracellular C allocation on growth rate that varies depending on the growth environment. (C) 2021 The Author(s). Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology.
Permanent Link: http://hdl.handle.net/11104/0325969