Actin Cytoskeleton Regulation by the Yeast NADPH Oxidase Yno1p Impacts Processes Controlled by MAPK Pathways

0549644 - MBÚ 2022 RIV CH eng J - Journal Article
Weber, M. - Basu, S. - Gonzalez, B. - Greslehner, G. P. - Singer, S. - Hašková, Danuša - Hašek, Jiří - Breitenbach, M. - Gourlay, C. W. - Cullen, P. J. - Rinnerthaler, M.
Actin Cytoskeleton Regulation by the Yeast NADPH Oxidase Yno1p Impacts Processes Controlled by MAPK Pathways.
Antioxidants. Roč. 10, č. 2 (2021), č. článku 322. E-ISSN 2076-3921
Institutional support: RVO:61388971
Keywords : ros * MAP kinase pathway * actin * osmotic stress * filamentous growth * invasive growth * pseudohyphal growth * pheromone response * apoptosis
OECD category: Microbiology
Impact factor: 7.675, year: 2021
Method of publishing: Open access
https://www.mdpi.com/2076-3921/10/2/322

Reactive oxygen species (ROS) that exceed the antioxidative capacity of the cell can be harmful and are termed oxidative stress. Increasing evidence suggests that ROS are not exclusively detrimental, but can fulfill important signaling functions. Recently, we have been able to demonstrate that a NADPH oxidase-like enzyme (termed Yno1p) exists in the single-celled organism Saccharomyces cerevisiae. This enzyme resides in the peripheral and perinuclear endoplasmic reticulum and functions in close proximity to the plasma membrane. Its product, hydrogen peroxide, which is also produced by the action of the superoxide dismutase, Sod1p, influences signaling of key regulatory proteins Ras2p and Yck1p/2p. In the present work, we demonstrate that Yno1p-derived H2O2 regulates outputs controlled by three MAP kinase pathways that can share components: the filamentous growth (filamentous growth MAPK (fMAPK)), pheromone response, and osmotic stress response (hyperosmolarity glycerol response, HOG) pathways. A key structural component and regulator in this process is the actin cytoskeleton. The nucleation and stabilization of actin are regulated by Yno1p. Cells lacking YNO1 showed reduced invasive growth, which could be reversed by stimulation of actin nucleation. Additionally, under osmotic stress, the vacuoles of a increment yno1 strain show an enhanced fragmentation. During pheromone response induced by the addition of alpha-factor, Yno1p is responsible for a burst of ROS. Collectively, these results broaden the roles of ROS to encompass microbial differentiation responses and stress responses controlled by MAPK pathways.
Permanent Link: http://hdl.handle.net/11104/0326021