Influence of different cytokinins on the phenolic acids and antioxidant activity of two Brachystelma species

0549509 - ÚEB 2022 RIV NL eng J - Journal Article
Hlophe, N. P. - Aremu, A.O. - Grúz, Jiří - Van Staden, J. - Finnie, J.F.
Influence of different cytokinins on the phenolic acids and antioxidant activity of two Brachystelma species.
Plant Cell, Tissue and Organ Culture. Roč. 145, č. 3 (2021), s. 689-699. ISSN 0167-6857. E-ISSN 1573-5044
Institutional support: RVO:61389030
Keywords : Apocynaceae * Conservation * Cytokinins * Flavonoids * Hydroxybenzoic acids * Hydroxycinnamic acids * Micropropagation * Phenolic acids
OECD category: Plant sciences, botany
Impact factor: 2.726, year: 2021
Method of publishing: Open access
http://doi.org/10.1007/s11240-021-02038-x

The genus Brachystelma morphologically ranges from slender climbers to geophytes that mainly occur in Australia, Southeast Asia and sub-Saharan Africa. These species are well-known for their nutrition and medicinal values. The current study evaluated the effect of cytokinins (CKs) on the phenolic acid content and antioxidant activity of two in vitro-regenerated Brachystelma species. We quantified the phenolic acids (using Ultra-high performance liquid chromatography-tandem mass spectrometry, UHPLC-MS/MS) and antioxidant (Oxygen radical absorbance capacity, ORAC) potential of two (B. pulchellum and B. pygmaeum) species that were successfully micropropagated, with the use of three cytokinins (CKs): benzyladenine (BA), isopentenyladenine (iP) and meta-topolin riboside (mTR). The presence of CKs strongly influenced the concentrations of the 11 phenolic acids (consisting of seven hydroxybenzoic acids and four hydroxycinnamic acids) and ORAC of the in vitro-regenerated B. pulchellum and B. pygmaeum. Generally, the levels of hydroxycinnamic acids were higher than the hydroxybenzoic acids in both Brachystelma species. Ferulic (9-55 mu g/g DW) and sinapic (14-97 mu g/g DW) acids were the major phenolic acids in the micro-propagated plants. In both Brachystelma species, the majority of the CK-regenerated plants had higher ORAC relative to the control. The best antioxidant activity (> 70 mu mol/g Trolox equivalents, TE) was obtained from 25 mu M iP and mTR treatments for B. pulchellum and B. pygmaeum, respectively. Overall, the current study demonstrated the ability of CKs to serve as a suitable tool for the production of valuable therapeutic chemicals in the two Brachystelma species.
Permanent Link: http://hdl.handle.net/11104/0325506