Concurrent overexpression of amino acid permease AAP1 (3a) and SUT1 sucrose transporter in pea resulted in increased seed number and changed cytokinin and protein levels

0549508 - ÚEB 2022 RIV US eng J - Journal Article
Grant, J. E. - Ninan, A. - Cripps-Guazzone, N. - Shaw, M. - Song, J. - Petřík, Ivan - Novák, Ondřej - Tegeder, M. - Jameson, P. E.
Concurrent overexpression of amino acid permease AAP1 (3a) and SUT1 sucrose transporter in pea resulted in increased seed number and changed cytokinin and protein levels.
Functional Plant Biology. Roč. 48, č. 9 (2021), s. 889-904. ISSN 1445-4408. E-ISSN 1445-4416
R&D Projects: GA ČR(CZ) GA18-07563S; GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : aap * cell wall invertase * ckx * ipt * legume * Pisum sativum * process peas * seed coat * seed yield * sucrose * sut * sweet * transgenic * transgenics
OECD category: Biochemistry and molecular biology
Impact factor: 2.812, year: 2021
Method of publishing: Open access
http://doi.org/10.1071/FP21011

Using pea as our model crop, we sought to understand the regulatory control over the import of sugars and amino acids into the developing seeds and its importance for seed yield and quality. Transgenic peas simultaneously overexpressing a sucrose transporter and an amino acid transporter were developed. Pod walls, seed coats, and cotyledons were analysed separately, as well as leaves subtending developing pods. Sucrose, starch, protein, free amino acids, and endogenous cytokinins were measured during development. Temporal gene expression analyses (RT-qPCR) of amino acid (AAP), sucrose (SUT), and SWEET transporter family members, and those from cell wall invertase, cytokinin biosynthetic (IPT) and degradation (CKX) gene families indicated a strong effect of the transgenes on gene expression. In seed coats of the double transgenics, increased content and prolonged presence of cytokinin was particularly noticeable. The transgenes effectively promoted transition of young sink leaves into source leaves. We suggest the increased flux of sucrose and amino acids from source to sink, along with increased interaction between cytokinin and cell wall invertase in developing seed coats led to enhanced sink activity, resulting in higher cotyledon sucrose at process pea harvest, and increased seed number and protein content at maturity.
Permanent Link: http://hdl.handle.net/11104/0325505