Biotechnological Conversion of Grape Pomace to Poly(3-hydroxybutyrate) by Moderately Thermophilic Bacterium Tepidimonas taiwanensis

0548661 - ÚPT 2022 RIV CH eng J - Journal Article
Kouřilová, X. - Pernicová, I. - Vidláková, M. - Krejčiřík, R. - Mrázová, Kateřina - Hrubanová, Kamila - Krzyžánek, Vladislav - Nebesářová, Jana - Obruča, S.
Biotechnological Conversion of Grape Pomace to Poly(3-hydroxybutyrate) by Moderately Thermophilic Bacterium Tepidimonas taiwanensis.
Bioengineering. Roč. 8, č. 10 (2021), č. článku 141. ISSN 2306-5354
R&D Projects: GA ČR(CZ) GA19-20697S; GA MŠMT(CZ) LM2015062
Institutional support: RVO:68081731 ; RVO:60077344
Keywords : polyhydroxyalkanoates * Tepidimonas taiwanensis * grape pomace * thermophiles
OECD category: Environmental biotechnology; Genetics and heredity (medical genetics to be 3) (BC-A)
Impact factor: 5.046, year: 2021
Method of publishing: Open access
https://www.mdpi.com/2306-5354/8/10/141

Polyhydroxyalkanoates (PHA) are microbial polyesters that have recently come to the forefront of interest due to their biodegradability and production from renewable sources. A potential increase in competitiveness of PHA production process comes with a combination of the use of thermophilic bacteria with the mutual use of waste substrates. In this work, the thermophilic bacterium Tepidimonas taiwanensis LMG 22826 was identified as a promising PHA producer. The ability to produce PHA in T. taiwanensis was studied both on genotype and phenotype levels. The gene encoding the Class I PHA synthase, a crucial enzyme in PHA synthesis, was detected both by genome database search and by PCR. The microbial culture of T. taiwanensis was capable of efficient utilization of glucose and fructose. When cultivated on glucose as the only carbon source at 50 °C, the PHA titers reached up to 3.55 g/L, and PHA content in cell dry mass was 65%. The preference of fructose and glucose opens the possibility to employ T. taiwanensis for PHA production on various food wastes rich in these abundant sugars. In this work, PHA production on grape pomace extracts was successfully tested.
Permanent Link: http://hdl.handle.net/11104/0324713