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Global Genome Mining Reveals a Cytochrome P450-Catalyzed Cyclization of Crownlike Cyclodipeptides with Neuroprotective Activity
- 1.0547817 - MBÚ 2022 RIV US eng J - Journal Article
Malit, J. J. L. - Liu, W. - Cheng, A. - Saha, Subhasish - Liu, L-L. - Qian, P.-Y.
Global Genome Mining Reveals a Cytochrome P450-Catalyzed Cyclization of Crownlike Cyclodipeptides with Neuroprotective Activity.
Organic Letters. Roč. 23, č. 17 (2021), s. 6601-6605. ISSN 1523-7060. E-ISSN 1523-7052
Institutional support: RVO:61388971
Keywords : identification * biosynthesis
OECD category: Microbiology
Impact factor: 6.072, year: 2021
Method of publishing: Limited access
https://pubs.acs.org/doi/10.1021/acs.orglett.1c01022
We conducted global genome mining of 162,672 bacterial genomes and identified 829 cyclodipeptide (CDP) biosynthesis gene clusters (BGC) containing a cytochrome P450 gene. Heterologous expression of BGC from Saccharopolyspora hirsuta DSM 44795 led to the identification of two novel crownlike CDPs, cyctetryptomycin A (4) and B (5), which possess unprecedented complex macrocycle and show neuroprotective activity. The two cytochrome P450s found in the BGC catalyze sequential reactions leading to the cyclization of diketopiperazine dimers.
Permanent Link: http://hdl.handle.net/11104/0323982
Number of the records: 1