Sphingolipids of plant pathogenic fungi

0547771 - MBÚ 2022 RIV CZ eng J - Journal Article
Gharwalová, L. - Kulišová, M. - Vasyliuk, A. - Marešová, Helena - Palyzová, Andrea - Nedbalová, L. - Kolouchová, I.
Sphingolipids of plant pathogenic fungi.
Plant Protection Science. Roč. 57, č. 2 (2021), s. 134-139. ISSN 1212-2580. E-ISSN 1805-9341
R&D Projects: GA MŠMT(CZ) LTC18009; GA ČR(CZ) GA19-10907S
Institutional support: RVO:61388971
Keywords : defensin * filamentous fungi * glycosylinositolphosphoceramides * high resolution tandem electrospray mass spectrometry * Vitis vinifera
OECD category: Microbiology
Impact factor: 1.414, year: 2021
Method of publishing: Open access
https://www.agriculturejournals.cz/web/pps.htm?type=article&id=131_2020-PPS

Glycosphingolipids in filamentous fungi are significant components of the plasma membrane and are vital for different cellular processes, such as growth, morphological transition or signal transduction. Fungal growth inhibitors targeting glycosylinositolphosphoceramide (GIPCs) biosynthesis or antifungal compounds binding to GIPCs present in membranes could present a safe way of preventing fungal growth on crops since GIPCs are not present in mammalian cells. Mass spectrometry-based shotgun lipidomics was used to analyze sphingolipids of 11 fungal strains isolated from plant material. Molecular species with inositol ceramides containing zero to five carbohydrates were identified. Differences in the amount of individual molecular species were influenced by the taxonomic affiliation. All tested strains exhibited a relatively high content (more than 40 mol.%) of GIPCs with three and more saccharides attached to the polar head. It could be assumed that the sphingolipid profiles of the tested plant pathogens would be an adaptation mechanism to antifungal plant defensins.
Permanent Link: http://hdl.handle.net/11104/0323952