Identification of a bio-signature for barley resistance against Pyrenophora teres infection based on physiological, molecular and sensor-based phenotyping

0547499 - ÚVGZ 2022 RIV IE eng J - Journal Article
Pandey, C. - Grosskinsky, D. K. - Westergaard, J. C. - Jorgensen, H. J. L. - Svensgaard, J. - Christensen, S. - Schulz, A. - Roitsch, Thomas
Identification of a bio-signature for barley resistance against Pyrenophora teres infection based on physiological, molecular and sensor-based phenotyping.
Plant Science. Roč. 313, DEC (2021), s. 1-11. ISSN 0168-9452. E-ISSN 1873-2259
R&D Projects: GA MŠMT(CZ) LO1415
Research Infrastructure: CzeCOS III - 90123
Institutional support: RVO:86652079
Keywords : source-sink relationships * carbohydrate-metabolism * hydrogen-peroxide * defense-mechanisms * leaf reflectance * gene-expression * theobroma-cacao * powdery mildew * stress * disease * Crop resistance * Enzyme activity signatures * Expression analysis * Multispectral imaging * Fungal DNA * PhenoLab * Pre-symptomatic * Bio-signatures
OECD category: Biochemistry and molecular biology
Impact factor: 5.363, year: 2021
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0168945221002685?via%3Dihub

Necrotic and chlorotic symptoms induced during Pyrenophora teres infection in barley leaves indicate a compatible interaction that allows the hemi-biotrophic fungus Pyrenophora teres to colonise the host. However, it is unexplored how this fungus affects the physiological responses of resistant and susceptible cultivars during infection. To assess the degree of resistance in four different cultivars, we quantified visible symptoms and fungal DNA and performed expression analyses of genes involved in plant defence and ROS scavenging. To obtain insight into the interaction between fungus and host, we determined the activity of 19 key enzymes of carbohydrate and antioxidant metabolism. The pathogen impact was also phenotyped non-invasively by sensor-based multireflectance andfluorescence imaging.
Permanent Link: http://hdl.handle.net/11104/0323714