Study of microbiocenosis of canine dental biofilms

0547342 - MBÚ 2022 RIV DE eng J - Journal Article
Kacirova, J. - Madari, A. - Mucha, R. - Kolesár Fecskeová, Lívia - Mujakić, Izabela - Koblížek, Michal - Nemcová, R. - Madar, M.
Study of microbiocenosis of canine dental biofilms.
Scientific Reports. Roč. 11, č. 1 (2021), č. článku 19776. ISSN 2045-2322. E-ISSN 2045-2322
Institutional support: RVO:61388971
Keywords : bacterial-flora * oral-health * plaque * nov. * cats * dogs
OECD category: Microbiology
Impact factor: 4.997, year: 2021
Method of publishing: Open access
https://www.nature.com/articles/s41598-021-99342-5

Dental biofilm is a complex microbial community influenced by many exogenous and endogenous factors. Despite long-term studies, its bacterial composition is still not clearly understood. While most of the research on dental biofilms was conducted in humans, much less information is available from companion animals. In this study, we analyzed the composition of canine dental biofilms using both standard cultivation on solid media and amplicon sequencing, and compared the two approaches. The 16S rRNA gene sequences were used to define the bacterial community of canine dental biofilm with both, culture-dependent and culture-independent methods. After DNA extraction from each sample, the V3-V4 region of the 16S rRNA gene was amplified and sequenced via Illumina MiSeq platform. Isolated bacteria were identified using universal primers and Sanger sequencing. Representatives of 18 bacterial genera belonging to 5 phyla were isolated from solid media. Amplicon sequencing largely expanded this information identifying in total 284 operational taxonomic units belonging to 10 bacterial phyla. Amplicon sequencing revealed much higher diversity of bacteria in the canine dental biofilms, when compared to standard cultivation approach. In contrast, cultured representatives of several bacterial families were not identified by amplicon sequencing.
Permanent Link: http://hdl.handle.net/11104/0323588