Thiolated poly(2-hydroxyethyl methacrylate) hydrogels as a degradable biocompatible scaffold for tissue engineering

0547091 - ÚMCH 2022 RIV CH eng J - Journal Article
Macková, Hana - Hlídková, Helena - Kaberova, Zhansaya - Proks, Vladimír - Kučka, Jan - Patsula, Vitalii - Vetrík, Miroslav - Janoušková, Olga - Podhorská, Bohumila - Pop-Georgievski, Ognen - Kubinová, Šárka - Horák, Daniel
Thiolated poly(2-hydroxyethyl methacrylate) hydrogels as a degradable biocompatible scaffold for tissue engineering.
Materials Science & Engineering C-Materials for Biological Applications. Roč. 131, December (2021), č. článku 112500. ISSN 0928-4931. E-ISSN 1873-0191
R&D Projects: GA ČR(CZ) GA20-07015S; GA ČR(CZ) GA20-07313S
Institutional support: RVO:61389013 ; RVO:68378041
Keywords : poly(2-hydroxyethyl methacrylate) * 2-methacryloyloxyethyl phosphorylcholine * 2-(acetylthio)ethyl methacrylate
OECD category: Polymer science; Biophysics (UEM-P)
Impact factor: 8.457, year: 2021
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0928493121006408

Research of degradable hydrogel polymeric materials exhibiting high water content and mechanical properties resembling tissues is crucial not only in drug delivery systems but also in tissue engineering, medical devices, and biomedical-healthcare sensors. Therefore, we newly offer development of hydrogels based on poly(2-hydroxyethyl methacrylate-co-2-(acetylthio) ethyl methacrylate-co-2-methacryloyloxyethyl phosphorylcholine) [P(HEMA-ATEMA-MPC)] and optimization of their mechanical and in vitro and in vivo degradability. P(HEMA-ATEMA-MPC) hydrogels differed in chemical composition, degree of crosslinking, and starting molar mass of polymers (15, 19, and 30 kDa). Polymer precursors were synthesized by a reversible addition fragmentation chain transfer (RAFT) polymerization using 2-(acetylthio)ethyl methacrylate containing protected thiol groups, which enabled crosslinking and gel formation. Elastic modulus of hydrogels increased with the degree of crosslinking (Slaughter et al., 2009) [1]. In vitro and in vivo controlled degradation was confirmed using glutathione and subcutaneous implantation of hydrogels in rats, respectively. We proved that the hydrogels with higher degree of crosslinking retarded the degradation. Also, albumin, γ-globulin, and fibrinogen adsorption on P(HEMA-ATEMA-MPC) hydrogel surface was tested, to simulate adsorption in living organism. Rat mesenchymal stromal cell adhesion on hydrogels was improved by the presence of RGDS peptide and laminin on the hydrogels. We found that rat mesenchymal stromal cells proliferated better on laminin-coated hydrogels than on RGDS-modified ones.
Permanent Link: http://hdl.handle.net/11104/0323584