Ecological Divergence Within the Enterobacterial Genus Sodalis: From Insect Symbionts to Inhabitants of Decomposing Deadwood

0546765 - MBÚ 2022 RIV CH eng J - Journal Article
Tláskal, Vojtěch - Pylro, Victor Satler - Žifčáková, Lucia - Baldrian, Petr
Ecological Divergence Within the Enterobacterial Genus Sodalis: From Insect Symbionts to Inhabitants of Decomposing Deadwood.
Frontiers in Microbiology. Roč. 12, JUN 11 2021 (2021), č. článku 668644. ISSN 1664-302X. E-ISSN 1664-302X
R&D Projects: GA ČR(CZ) GC21-09334J
Institutional support: RVO:61388971
Keywords : insect symbionts * Sodalis * deadwood * nitrogen fixation * free-living * non-symbiotic
OECD category: Microbiology
Impact factor: 6.064, year: 2021
Method of publishing: Open access
https://www.frontiersin.org/articles/10.3389/fmicb.2021.668644/full

The bacterial genus Sodalis is represented by insect endosymbionts as well as free-living species. While the former have been studied frequently, the distribution of the latter is not yet clear. Here, we present a description of a free-living strain, Sodalis ligni sp. nov., originating from decomposing deadwood. The favored occurrence of S. ligni in deadwood is confirmed by both 16S rRNA gene distribution and metagenome data. Pangenome analysis of available Sodalis genomes shows at least three groups within the Sodalis genus: deadwood-associated strains, tsetse fly endosymbionts and endosymbionts of other insects. This differentiation is consistent in terms of the gene frequency level, genome similarity and carbohydrate-active enzyme composition of the genomes. Deadwood-associated strains contain genes for active decomposition of biopolymers of plant and fungal origin and can utilize more diverse carbon sources than their symbiotic relatives. Deadwood-associated strains, but not other Sodalis strains, have the genetic potential to fix N-2, and the corresponding genes are expressed in deadwood. Nitrogenase genes are located within the genomes of Sodalis, including S. ligni, at multiple loci represented by more gene variants. We show decomposing wood to be a previously undescribed habitat of the genus Sodalis that appears to show striking ecological divergence.
Permanent Link: http://hdl.handle.net/11104/0323152