Protein corona inhibits endosomal escape of functionalized DNA nanostructures in living cells

0546722 - FZÚ 2022 RIV US eng J - Journal Article
Smolková, Barbora - MacCulloch, T. - Rockwood, T.F. - Liu, M. - Henry, S.J.W. - Frtús, Adam - Uzhytchak, Mariia - Lunova, Mariia - Hof, Martin - Jurkiewicz, Piotr - Dejneka, Alexandr - Stephanopoulos, N. - Lunov, Oleg
Protein corona inhibits endosomal escape of functionalized DNA nanostructures in living cells.
ACS Applied Materials and Interfaces. Roč. 13, č. 39 (2021), s. 46375-46390. ISSN 1944-8244. E-ISSN 1944-8252
R&D Projects: GA MŠMT LTC19040; GA ČR(CZ) GX19-26854X
Institutional support: RVO:68378271 ; RVO:61388955
Keywords : nanotechnology * DNA nanotechnology * cellular uptake * protein corona * bionano interactions * endolysosomal escape
OECD category: Biophysics; Physical chemistry (UFCH-W)
Impact factor: 10.383, year: 2021
Method of publishing: Limited access
https://doi.org/10.1021/acsami.1c14401

DNA nanostructures (DNs) can be designed in a controlled and programmable manner, and these structures are increasingly used in a variety of biomedical applications, such as the delivery of therapeutic agents. When exposed to biological liquids, most nanomaterials become covered by a protein corona, which in turn modulates their cellular uptake and the biological response they elicit. However, the interplay between living cells and designed DNs are still not well established. Namely, there are very limited studies that assess protein corona impact on DN biological activity. Here, we analyzed the uptake of functionalized DNs in three distinct hepatic cell lines.
Permanent Link: http://hdl.handle.net/11104/0323188