Development of CE-C4d method for determination tropane alkaloids

0546255 - ÚIACH 2022 RIV CH eng J - Journal Article
Gołab, M. - Przybyłowska, M. - Kubáň, Petr - Itterheimová, Petra - Wózniakiewicz, M.
Development of CE-C4d method for determination tropane alkaloids.
Molecules. Roč. 26, č. 19 (2021), č. článku 5749. E-ISSN 1420-3049
Institutional support: RVO:68081715
Keywords : capillary electrophoresis * tropane alkaloids * conductivity detection
OECD category: Analytical chemistry
Impact factor: 4.927, year: 2021
Method of publishing: Open access
https://www.mdpi.com/1420-3049/26/19/5749

A fast method for the determination of tropane alkaloids, using a portable CE instrument with a capacitively coupled contactless conductivity detector (CE-C4D) was developed and validated for determination of atropine and scopolamine in seeds from Solanaceae family plants. Sepa-ration was obtained within 5 min, using an optimized background electrolyte consisting of 0.5 M acetic acid with 0.25% (w/v) β-CD. The limit of detection and quantification was 0.5 µg/mL and 1.5 µg/mL, respectively, for both atropine and scopolamine. The developed method was validated with the following parameters—precision (CV): 1.07–2.08%, accuracy of the assay (recovery, RE): 101.0– 102.7% and matrix effect (ME): 92.99–94.23%. Moreover, the optimized CE-C4D method was applied to the analysis of plant extracts and pharmaceuticals, proving its applicability and accuracy.
Permanent Link: http://hdl.handle.net/11104/0322804