Epitachophoretic preconcentration of proteins

0546004 - ÚIACH 2022 RIV CZ eng C - Conference Paper (international conference)
Hrušková, Helena - Voráčová, Ivona - Foret, František
Epitachophoretic preconcentration of proteins.
Proceedings of the 17th International Students Conference “Modern Analytical Chemistry”. Praha: Přírodovědecká fakulta Univerzity Karlovy, 2021 - (Nesměrák, K.), s. 170-175. ISBN 978-80-7444-089-2.
[International Students Conference 'Modern Analytical Chemistry' /17./. Praha (CZ), 16.09.2021-17.09.2021]
Institutional support: RVO:68081715
Keywords : epitachophoresis * isotachophoresis * preparative concentration
OECD category: Analytical chemistry

In this work, we present a developed method for preconcentration of standards of proteins cytochrome c, myoglobin, and hemoglobin. Firstly, the electrolyte system was selected and optimized to provide low analysis time, narrow shape of the protein zone, and eliminate overheating at the center of the device. In the next step, the developed method was tested on the preconcentration of proteins: cytochrome c, myoglobin, and hemoglobin. After several adjustments myoglobin and hemoglobin were also uccessfully preconcentrated. After evaluation of pilot results, this technique provides up to fiftyfold preconcentration and recovery of over 70 % for each protein. The next plan is to preconcentrate proteins from complex biological matrices such as urine or blood plasma.
Permanent Link: http://hdl.handle.net/11104/0322598