Insights into the role of transcriptional gene silencing in response to herbicide‐treatments in arabidopsis thaliana

0545881 - ÚEB 2022 RIV CH eng J - Journal Article
Markus, C. - Pečinka, Aleš - Merotto, A.
Insights into the role of transcriptional gene silencing in response to herbicide‐treatments in arabidopsis thaliana.
International Journal of Molecular Sciences. Roč. 22, č. 7 (2021), č. článku 3314. E-ISSN 1422-0067
R&D Projects: GA MŠMT(CZ) EF16_019/0000827
Grant - others:AVČR(CZ) Purkyně Fellowship
Institutional support: RVO:61389030
Keywords : 2,4‐d * Chromatin mutants * Epigenetics * Glyphosate * Herbicide resistance * Imazethapyr * ros1
OECD category: Biochemistry and molecular biology
Impact factor: 6.208, year: 2021
Method of publishing: Open access
http://doi.org/10.3390/ijms22073314

Herbicide resistance is broadly recognized as the adaptive evolution of weed populations to the intense selection pressure imposed by the herbicide applications. Here, we tested whether transcriptional gene silencing (TGS) and RNA‐directed DNA Methylation (RdDM) pathways mod-ulate resistance to commonly applied herbicides. Using Arabidopsis thaliana wild‐type plants exposed to sublethal doses of glyphosate, imazethapyr, and 2,4‐D, we found a partial loss of TGS and increased susceptibility to herbicides in six out of 11 tested TGS/RdDM mutants. Mutation in RE‐ PRESSOR OF SILENCING 1 (ROS1), that plays an important role in DNA demethylation, leading to strongly increased susceptibility to all applied herbicides, and imazethapyr in particular. Tran-scriptomic analysis of the imazethapyr‐treated wild type and ros1 plants revealed a relation of the herbicide upregulated genes to chemical stimulus, secondary metabolism, stress condition, flavonoid biosynthesis, and epigenetic processes. Hypersensitivity to imazethapyr of the flavonoid bio-synthesis component TRANSPARENT TESTA 4 (TT4) mutant plants strongly suggests that ROS1‐ dependent accumulation of flavonoids is an important mechanism for herbicide stress response in A. thaliana. In summary, our study shows that herbicide treatment affects transcriptional gene silencing pathways and that misregulation of these pathways makes Arabidopsis plants more sensitive to herbicide treatment.
Permanent Link: http://hdl.handle.net/11104/0322507